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Dr P Mahendra


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Be Part of Research - Trial Details - Effect of chemotherapy and ionising radiation on sperm nuclear and mitochondrial DNA: Can pre-treatment with GnRH Agonists reverse these effects?
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Effect of chemotherapy and ionising radiation on sperm nuclear and mitochondrial DNA: Can pre-treatment with GnRH Agonists reverse these effects?

Not Recruiting

Open to: Male

Age: Adult

Birmingham

Medical Conditions

Urological and Genital Diseases

This information is provided directly by researchers and we recognise that it isn't always easy to understand. We are working with researchers to improve the accessibility of this information.


Not provided at time of registration

Start dates may differ between countries and research sites. The research team are responsible for keeping the information up-to-date.  

The recruitment start and end dates are as follows:

01 Oct 2003 01 Jan 2007

Interventional

Intervention Type : Other
Intervention Description : The investigations will comprise the following:

Sperm StorageImmediately on referral, patients will be given the opportunity to have spermatozoa cryopreserved at the ACU, Birmingham Women's Hospital. Briefly, after a full semen analysis (see below) and completion of relevant documentation, an equal amount of cryoprotectant media is added to the semen over a period of 10-15 minutes. Vials are then suspended in liquid nitrogen vapour.

Semen AnalysisFull semen analysis, including sperm concentration; motility; morphology; antisperm antibodies. vitality are carried out in accordance with the World Health organisation (WHO, 1992). Computer assisted sperm motility analysis (CASA) will also be performed, using an Hamilton Thorn IVOS (version 8.1).(Tomlinson Ct al, 1993).

Blood testsBloods for serum FSH and testosterone will be taken at the time of semen analyses.Sperm nuclear DNA (nDNA) and mitochondrial DNA (mtDNA)Sperm nuclear DNA Damage will be assessed using the TUNEL assay or using the sperm nuclearcliromatin integrity analysed using the Chromomycin A3 fluorochrome (Manicardi et al, 1995; 1998) Mitochondrial DNA fragmentation will be studied using long PCR according to the methods of St.John, (in press).

Mitochondrial FunctionMitochondrial membrane potential will be assessed using the fluorescent probe D1Oc6 counterstained with propidium iodide for sperm viability according to the methods Zamzami et al (1996).

SamplesSamples will be assessed immediately after referral from the oncology centres. A second sample will be assessed 3 months later and then again at 6 months.

All the above mentioned techniques have been developed and validated and are in current use in our laboratories.




You can take part if:



You may not be able to take part if:


Not provided at time of registration


Below are the locations for where you can take part in the trial. Please note that not all sites may be open.

  • Clinical Haematology
    Birmingham
    B15 2TH

This information has not yet been provided by the study team. You'll have an opportunity to discuss any risks and benefits that may be associated with this study prior to consenting to taking part.

Dr P Mahendra



The study is sponsored by Department of Health and funded by University Hospital Birmingham NHS Trust (UK); Research Funds.



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Read full details for Trial ID: ISRCTN77877146
Last updated 13 March 2014

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